期刊
RESEARCH IN MICROBIOLOGY
卷 164, 期 2, 页码 127-135出版社
ELSEVIER
DOI: 10.1016/j.resmic.2012.10.010
关键词
pDU1; pRL25; Relative plasmid copy number; Phenotypic variation; GAF domain; Cyanobacterium
类别
资金
- National Natural Science Foundation of China [30670046, 30970088]
The cyanobacterium Anabaena (Nostoc) sp. PCC 7120 is a model for cyanobacterial cell differentiation studies. pDU1, an endogenous plasmid in Nostoc sp. PCC 7524, is used as the only cyanobacterial replicon for Anabaena (Nostoc) studies. However, the relative copy numbers of pDU1-based plasmids in Anabaena (Nostoc) sp. PCC 7120 are not well studied. We found that the relative plasmid copy number of one such vector, pRL25T, varied widely, especially when the vector carried a recombinant insert, under different conditions, ranging from 0.53 to 1812 per chromosome in different recombinant strains tested, either in independent clones of the same strain or in the same clone under different growth conditions. The phenotypes caused by pRL25T-driven expression of green fluorescent protein or the GAF domain of Pkn41 or Pkn42 varied depending on the independent clones analyzed. This phenotypic variation correlated with the relative plasmid copy number present in cells. (C) 2012 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
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