期刊
RESEARCH IN MICROBIOLOGY
卷 164, 期 7, 页码 749-760出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.resmic.2013.04.001
关键词
AHL mimic; Biosensor; Lactonase; Quorum sensing; Quorum quenching; Biofilm
类别
资金
- Spanish Ministerio de Ciencia y Tecnologia [AGL2009-13487-C04]
- Junta de Andalucia, Consejeria de Innovacion, Ciencia y Empresas [AGR-5821]
- FPU fellowship from the Spanish Ministerio de Ciencia y Tecnologia
Many bacteria regulate their gene expression in response to changes in their population density in a process called quorum sensing (QS), which involves communication between cells mediated by small diffusible signal molecules termed autoinducers. N-acyl-homoserine-lactones (AHLs) are the most common autoinducers in proteobacteria. QS-regulated genes are involved in complex interactions between bacteria of the same or different species and even with some eukaryotic organisms. Eukaryotes, including plants, can interfere with bacterial QS systems by synthesizing molecules that interfere with bacterial QS systems. In this work, the presence of AHL-mimic QS molecules in diverse Oryza sativa (rice) and Phaseolus vulgaris (bean) plant-samples were detected employing three biosensor strains. A more intensive analysis using biosensors carrying the lactonase enzyme showed that bean and rice seed-extract contain molecules that lack the typical lactone ring of AHLs. Interestingly, these molecules specifically alter the QS-regulated biofilm formation of two plant-associated bacteria, Sinorhizobium fredii SMH12 and Pantoea ananatis AMG501, suggesting that plants are able to enhance or to inhibit the bacterial QS systems depending on the bacterial strain. Further studies would contribute to a better understanding of plant bacteria relationships at the molecular level. (C) 2013 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
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