期刊
RESEARCH IN MICROBIOLOGY
卷 163, 期 6-7, 页码 479-486出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.resmic.2012.06.001
关键词
Arenicin-1; Arenicola marina; Antimicrobial peptide; Synergistic effect; Hydroxyl radical; Bacterial membrane
类别
资金
- Marine and Extreme Genome Research Center Program of the Ministry of Land, Transportation and Maritime Affairs, Republic of Korea
- Korea Institute of Marine Science & Technology Promotion (KIMST) [20046001] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Arenicin-1, a 21-mer antimicrobial peptide, has been known to exert broad bactericidal activity. In this study, the combination effect of arenicin-1 with conventional antibiotics was investigated and all combinations showed synergistic effects against bacterial strains (fractional inhibitory concentration index <= 0.75). In an assay using fluorescent dye 3'-(p-hydroxyphenyl) fluorescein (HPF) and hydroxyl radical scavenger thiourea, we demonstrated that combined treatments of arenicin-1 and antibiotics caused synergistic effects by producing hydroxyl radicals, particularly in bacterial strains treated with the peptide and ampicillin. The oxidative stress induced by arenicin-1 was stimulated by transient depletion of NADH. Flow cytometric analysis with propidium iodide (PI) indicated that MIC of arenicin-1 for combination continued to increase the permeability of the bacterial cytoplasmic membrane, enhancing the entry of erythromycin and chloramphenicol which act as protein synthesis inhibitors. Therefore, arenicin-1 synergizes with antibiotics by means of hydroxyl radical formation or membrane-active mechanisms. This combination therapy allows the use of lower concentrations of arenicin-1 and restores the effectiveness of antibiotics. (c) 2012 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
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