期刊
RESEARCH IN MICROBIOLOGY
卷 162, 期 3, 页码 240-248出版社
ELSEVIER
DOI: 10.1016/j.resmic.2010.11.008
关键词
Escherichia coli O157:H7; rfb(O157); fliC(H7); Cattle; Pigs; Humans
类别
资金
- North-West University
- Faculty of Agriculture, Science and Technology
The aim of the present study was to isolate and identify Escherichia coli O157:H7 from pigs, cattle, humans, beef, pork and water samples and to determine their putative virulence genes by PCR analysis. A total of 220 samples were analysed; 5600 presumptive E. coli 0157:H7 were screened for the presence of rfb(O157) and fliC(H7) gene fragments by PCR and 130 isolates were confirmed. The prevalence of E. coli 0157:H7 was higher in pigs and pork 88(67.7%) than in cattle and beef 36(27.7%), water 3(2.3%) or humans 1 (0.77%). Moreover, the pathogen was more frequently isolated from faecal (16.9%-43.1%) than from meat samples (I0.8%-24.6%). A large proportion-73 (56.2%) of the isolates possessed the hlyA gene, while 48 (36.9%) harboured the eaeA gene. Although there were no major differences in the number of isolates harbouring the stx(1) and stx(2) genes, respectively, only a small proportion 13(10%) harboured both shiga toxin genes. Despite this, the proportion of isolates that possessed the stx(1) 29(22.3%) was higher than those possessing the stx2 gene. None of the E. coli 0157:H7 isolates harboured all four shiga-toxin producing E. coli (STEC) virulence genes investigated. When comparing the proportion of isolates obtained from the different sample sources and/or stations, significant positive correlations were observed between isolates from Mafikeng and Lichtenburg (r = 0.981, p <0.05) and those from Mafikeng and Rustenburg (r = 0.991, p <0.05). These results therefore indicate that meat and faeces samples obtained from major cities in the northwest province were contaminated with E. coli 0157:H7. We suggest that there is a need for improving the sanitary conditions of farms, abattoirs and butcher shops. This could reduce transmission of E. coli 0157:H7 to humans. (C) 2011 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
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