期刊
RESEARCH IN MICROBIOLOGY
卷 162, 期 7, 页码 701-714出版社
ELSEVIER
DOI: 10.1016/j.resmic.2011.06.009
关键词
Daphnia; Collagen; Bacterial proteins; Virulence; Pasteuria
类别
资金
- Swiss National Science Foundation
- Freiwillige Akademische Geselschaft, Basel University
- Australian Postgraduate Award
Collagen-like proteins containing glycine-X-Y repeats have been identified in several pathogenic bacteria potentially involved in virulence. Recently, a collagen-like surface protein, Pclla, was identified in Pasteuria ramosa, a spore-forming parasite of Daphnia. Here we characterise 37 novel putative P. ramosa collagen-like protein genes (PCLs). PCR amplification and sequencing across 10 P. ramosa strains showed they were polymorphic, distinguishing genotypes matching known differences in Daphnia/P. ramosa interaction specificity. Thirty PCLs could be divided into four groups based on sequence similarity, conserved N- and C-terminal regions and G-X-Y repeat structure. Group 1, Group 2 and Group 3 PCLs formed triplets within the genome, with one member from each group represented in each triplet. Maximum-likelihood trees suggested that these groups arose through multiple instances of triplet duplication. For Group 1, 2, 3 and 4 PCLs, X was typically proline and Y typically threonine, consistent with other bacterial collagen-like proteins. The amino acid composition of Pcl2 closely resembled Pclla, with X typically being glutamic acid or aspartic acid and Y typically being lysine or glutamine. Pcl2 also showed sequence similarity to Pclla and contained a predicted signal peptide, cleavage site and transmembrane domain, suggesting that it is a surface protein. (C) 2011 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
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