期刊
RESEARCH IN MICROBIOLOGY
卷 162, 期 5, 页码 542-549出版社
ELSEVIER
DOI: 10.1016/j.resmic.2011.04.009
关键词
Antibactericidal mechanism; Muramic acid; Peptidoglycan; Silver nanoparticles; Staphylococcus aureus
类别
资金
- Research Council of Shahid Beheshti University
- Iran Nanotechnology Initiative Council
Antibacterial activity of silver nanoparticles (AgNPs) was investigated using Staphylococcus aureus PTCC1431 as a model of Gram-positive bacteria. The mechanism of antibacterial activity of AgNPs was then studied by analyzing the growth, morphology, and molecular variations in the cell wall. Experimental data showed that AgNPs at a concentration of 4 mu g/ml completely inhibited bacterial growth. Transmission electron microscopy results confirmed cell wall damage produced by AgNPs as well as accumulation of AgNPs in the bacterial membrane. Meanwhile, the AgNP-treated bacteria were monitored by circular dichroism to reveal peptidoglycan variations. Some degree of variation in the alpha-helix position of the peptide chain was observed. Moreover, increasing the AgNP concentration to 8 mu g/ml resulted in release of muramic acid (MA) into the medium, which could be attributed to cell wall distraction. A gas chromatography-tandem mass spectrometry analysis and release of MA, as a bacterial indicator, showed that glycan strands may also be decomposed as a result of AgNP treatment. (C) 2011 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
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