期刊
RESEARCH IN MICROBIOLOGY
卷 159, 期 7-8, 页码 516-521出版社
ELSEVIER
DOI: 10.1016/j.resmic.2008.06.007
关键词
Methyl-coenzyme M reductase; Human intestinal microflora; Methanogens; Gut Microbiota
类别
The diversity of methanogenic Archaea from the gut of 6 humans was investigated by targeting mcrA, a molecular metabolic marker of methanogenesis. Three operational taxonomic units (OTUs) were recovered from about 400 clones analyzed, two of which were attributed to the expected Methanobacteriales Methanobrevibacter smithii (4 volunteers) and Methanosphaera stadtmanae (1 volunteer). The third OTU (1 Volunteer) corresponded to a distant phylotype that does not cluster with any of the five methanogenic orders. This result, also supported by 16S archaeal sequences retrieved from the same volunteer, strongly suggests there may be a sixth order and hence potential underestimation of the role of methanogens in gut physiology. (C) 2008 Elsevier Masson SAS. All rights reserved.
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