期刊
REPRODUCTIVE TOXICOLOGY
卷 46, 期 -, 页码 1-11出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.reprotox.2014.02.008
关键词
Melamine; Cyanuric acid; Transmission electron microscopy; Blood-testis barrier; Apoptosis; Vimentin
资金
- National Natural Science Foundation of China [31272515]
- Research Foundation for the Doctoral Program of Higher Education of China [20130008110030]
Eight-week-old male Kunming mice were administered either melamine (MA, 30,140, or 700 mg/kg/day), a melamine and cyanuric acid mixture (MC, each at 15, 70, or 350 mg/kg/day), or vehicle (control) for 3 consecutive days. Testicular toxicity was evaluated on days 1 and 5 after the final exposure. The testicular and epididymal weights and serum testosterone level were significantly decreased in the highest MC group (350 mg/kg/day). Histopathologically, both MA and MC caused obvious lesions in the testis and epididymis, with significant increases in sperm abnormalities. By TEM, the blood-testis barrier was damaged dose dependently. TUNEL staining showed that both MA and MC induced increases in germ cell apoptosis. The Sertoli cell vimentin was collapsed in the treated animals as detected by immunohistochemical staining and Western blotting. This study demonstrated that both MA and MC treatments could disrupt the blood-testis barrier and cause a clear testicular toxicity. (C) 2014 Elsevier Inc. All rights reserved.
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