期刊
REPRODUCTIVE TOXICOLOGY
卷 38, 期 -, 页码 81-88出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.reprotox.2013.03.005
关键词
Sertoli cells; 2,4-Dichlorophenoxyacetic acid; Spermatogenesis; Glucose metabolism
资金
- Fundacao para a Ciencia e a Tecnologia - FCT [PTDC/QUI-BIQ/121446/2010, PEst-C/SAU/UI0709/2011]
- Fundo Europeu de Desenvolvimento Regional - FEDER via Programa Operacional Factores de Competitividade - COMPETE/QREN
- FCT [SFRH/BPD/80451/2011]
- FCT through FSE fund (Programa Ciencia)
- FCT through POPH fund (Programa Ciencia)
The purpose of this study was to determine the effects of 2,4-D, an herbicide used worldwide also known as endocrine disruptor, in Sertoli cell (SC) metabolism. Immature rat SCs were maintained 50 h under basal conditions or exposed to 2,4-D (100 nM, 10 mu M and 1 mM). SCs exposed to 10 mu M and 1 mM of 2,4-D presented lower intracellular glucose and lactate content. Exposure to 10 mu M of 2,4-D induced a significant decrease in glucose transporter-3 mRNA levels and phosphofructokinase-1 mRNA levels decreased in cells exposed to 100 nM and 10 mu M of 2,4-D. Exposure to 100 nM and 10 mu M also induced a decrease in lactate dehydrogenase (LDH) mRNA levels while the LDH protein levels were only decreased in cells exposed to 1 mM of 2,4-D. Exposure to 2,4-D altered glucose uptake and metabolization in SCs, as well as lactate metabolism and export that may result in impaired spermatogenesis. (c) 2013 Elsevier Inc. All rights reserved.
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