The effect of triclosan on hormone secretion and viability of human choriocarcinoma JEG-3 cells

Triclosan is an antimicrobial agent frequently used in pharmaceuticals and personal care products. We analyzed triclosan for its action on placental secretion of progesterone, estradiol and human chorionic gonadotropin in vitro in the JEG-3 cells. We also investigated its action on cell viability, proliferation and apoptosis. The JEG-3 cells were cultured with increasing doses of triclosan (1 x 10(-9)-1 x 10(-4) M) for 24, 48 and 72 h. Triclosan was found to increase estradiol and progesterone secretion after short- and long-term exposure. The stimulatory effect was observed up to 10 mu M after short- and long-term exposure to triclosan. In addition, triclosan caused an adverse effect on beta-hCG secretion. The highest doses of triclosan (50 and 100 mu M) showed a strong cytotoxic effect. Anti proliferative and pro-apoptotic effects were also observed. Overall, this study demonstrates that triclosan may indirectly disrupt steroidogenesis which may, in turn, affect placental development and consequently fetal growth. (C) 2012 Elsevier Inc. All rights reserved.