期刊
REPRODUCTIVE SCIENCES
卷 17, 期 4, 页码 366-376出版社
SAGE PUBLICATIONS INC
DOI: 10.1177/1933719109355196
关键词
myometrium; labor; Na; K-ATPase; contraction; smooth muscle; isoform; FXYD1; FXYD2; immunohistochemistry; RT-PCR
资金
- The Wellcome Trust, United Kingdom [065559/Z/01/]
Na, K-ATPase activity relies on the composition of its catalytic alpha, beta, and FXYD constituents, all of which are expressed as multiple isoforms (4 alpha, 4 beta, and 7 FXYD). We used reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry to study Na, K-ATPase expression in uterine samples from nonlaboring elective and laboring emergency caesarean sections (CSs). Transcripts of alpha 1 to 3, beta 1 to 3, and FXYD1 isoforms were detected in all samples, but FXYD2 was only present in hysterectomy samples. Abundant immunoreactivity of alpha 1 and moderate alpha 2 was localized in myometrial smooth muscle and secretory glands of all groups. Smooth muscle and gland epithelia showed diffuse cytoplasmic alpha 3 immunoreactivity. beta isoforms were detected in all groups but beta 3 showed much denser immunoreactivity in myometrial samples taken from women in labor. In pregnancy, there was a switch in isoform expression, resulting in increased beta 3 and decreased FXYD2 at the protein and messenger RNA (mRNA) levels. Na, K-ATPase isoform alterations may modulate uterine contractility during labor.
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