期刊
REPRODUCTIVE BIOMEDICINE ONLINE
卷 26, 期 1, 页码 68-78出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.rbmo.2012.09.019
关键词
Comet assay; ICSI; IVF; male infertility; sperm DNA damage
资金
- Hamilton Thorne Biosciences Ltd.
- Ulster Garden Villages
Sperm DNA damage has a negative impact on pregnancy rates following assisted reproduction treatment (ART). The aim of the present study was to examine the relationship between sperm DNA fragmentation and live-birth rates after IVF and intracytoplasmic sperm injection (ICSI). The alkaline Comet assay was employed to measure sperm DNA fragmentation in native semen and in spermatozoa following density-gradient centrifugation in semen samples from 203 couples undergoing IVF and 136 couples undergoing ICSI. Men were divided into groups according to sperm DNA damage. Following IVF, couples with <25% sperm DNA fragmentation had a live-birth rate of 33%; in contrast, couples with >50% sperm DNA fragmentation had a much lower live-birth rate of 13%. Following ICSI, no significant differences in sperm DNA damage were found between any groups of patients. Sperm DNA damage was also associated with low live-birth rates following IVF in both men and couples with idiopathic infertility: 39% of couples and 41% of men with idiopathic infertility have high sperm DNA damage. Sperm DNA damage assessed by the Comet assay has a close inverse relationship with live-birth rates after IVF. RBMOnline (C) 2012, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.
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