期刊
REPRODUCTIVE BIOMEDICINE ONLINE
卷 17, 期 5, 页码 642-651出版社
ELSEVIER SCI LTD
DOI: 10.1016/S1472-6483(10)60311-2
关键词
activin; HCG; inhibin; protein kinase; QRT-PCR
资金
- National Health and Medical Research Council [211244]
Ovulation is associated with a rise in activin A and a decline in pro-alpha C, inhibin A and inhibin B secretion. It is believed that the actions of inhibin and activin during human chorionic gonadotrophin (HCG) stimulation are mediated by protein kinase A (PKA) and/or protein kinase C (PKC). Using ail in-vitro murine preantal follicle culture model, the effects of it PKA inhibitor, Rp-cAMP, and a PKC inhibitor. PKIM, on inhibin and activin gene expression, secretion, ovulation and oocyte maturation were studied during HCG stimulation. Both Rp-cAMP (0.1 mu mol/l and 1.0 mu mol/l) and PKIM (1.0 mu mol/l) significantly (P < 0.001) inhibited the action of HCG by suppressing the increase in activin A secretion whilst preventing the decline in pro-alpha C, inhibin A and B. In addition, Rp-cAMP and PKIM were able to significantly (P < 0.05) reduce the rate of HCG-induced ovulation and meiotic resumption, but had no effect on the completion of oocyte maturation. Furthermore, HCG-induced ovulation resulted in the reduction of all three inhibin subunits, but inhibin subunit expression was not affected by Rp-cAMP and PKIM. These results provide evidence supporting a role for PKA and PKC pathways in the signalling mechanism for inhibin and activin action during ovulation and meiotic resumption of the oocyte.
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