期刊
REPRODUCTION
卷 145, 期 3, 页码 277-288出版社
BIOSCIENTIFICA LTD
DOI: 10.1530/REP-12-0435
关键词
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资金
- Ministry of Education, Science, Sports and Culture of Japan [20062009]
- Grants-in-Aid for Scientific Research [24890300, 20062009] Funding Source: KAKEN
Somatic cell nuclear transfer, a technique used to generate clone embryos by transferring the nucleus of a somatic cell into an enucleated oocyte, is an excellent approach to study the reprogramming of the nuclei of differentiated cells. Here, we conducted a transcriptomic study by performing microarray analysis on single Sertoli cell nuclear transfer (SeCNT) embryos throughout preimplantation development. The extensive data collected from the oocyte to the blastocyst stage helped to identify specific genes that were incorrectly reprogrammed at each stage, thereby providing a novel perspective for understanding reprogramming progression in SeCNT embryos. This attempt provided an opportunity to discuss the possibility that ectopic gene expression could be involved in the developmental failure of SeCNT embryos. Network analysis at each stage suggested that in total, 127 networks were involved in developmental and functional disorders in SeCNT embryos. Furthermore, chromosome mapping using our time-lapse expression data highlighted temporal-spatial changes of the abnormal expression, showing the characteristic distribution of the genes on each chromosome. Thus, the present study revealed that the preimplantation development of SeCNT embryos appears normal; however, the progression of incorrect reprogramming is concealed throughout development. Reproduction (2013) 145 277-288
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