期刊
REPRODUCTION
卷 138, 期 6, 页码 953-960出版社
BIOSCIENTIFICA LTD
DOI: 10.1530/REP-09-0235
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资金
- Natural Sciences and Engineering Research Council of Canada
- Grayson-Jockey Club Research Foundation
- Equine Guelph
- Ontario Ministry of Agriculture, Food and Rural Affairs
Estrogen production by the trophoblast is considered important in early equine pregnancy and leads to high concentrations in yolk-sac (Y-S) fluid. The embryo proper is a potential site for their action. We examined estrogen metabolism in the embryo proper because some actions of estrogens are derived from locally formed metabolites. The embryo proper, as well as separated extraembryonic tissues, of conceptuses collected about day 25 of pregnancy, were incubated with (3)[H]-estrone (E-1) and (3)[H]-estradiol (E-2). Steroids were recovered from media by solid-phase extraction and eluted separately as unconjugated and conjugated fractions. Profiles of free and sulfo-conjugated fractions were obtained by HPLC. Some differences and similarities were noted for the embryo proper as compared to the extraembryonic tissues. No reduction of E-1 to E-2 was noted for the embryo proper and allantois, but some was seen with the bilaminar Y-S wall. Less conversion of E-2 to E-1 occurred in the embryo proper than in the extraembryonic tissues. Profiles for hydrolyzed sulfates from incubation of the embryo proper were very similar for both substrates, mainly with E-1 present. Thus, low levels of reductase and high levels of oxido-activities were apparent for the 17 beta-hydroxysteroid dehydrogenase enzymes. Further evidence of an active role for the embryo proper was seen as minor, polar products, and an unknown compound eluting between E-2 and E-1. These findings show, for the first time, that the embryo proper can metabolize estrogens that are found in Y-S fluid - a function of potential significance at this stage in its development. Reproduction (2009) 138 953-960
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