期刊
RENEWABLE ENERGY
卷 66, 期 -, 页码 625-633出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.renene.2013.12.032
关键词
Glycerol; Bioethanol; Optimization; Response surface methodology; Escherichia coli SS1
资金
- Research University Grants (RUGS)
- Universiti Putra Malaysia and Science Fund
- Ministry of Science, Technology & Innovation, Malaysia (MOSTI)
Bioethanol is a promising biofuel and has a lot of great prospective and could become an alternative to fossil fuels. Ethanol fermentation using glycerol as carbon source was carried out by local isolate, ethanologenic bacterium Escherichia coli SS1 in a close system. Factors affecting bioethanol production from pure glycerol were optimized via response surface methodology (RSM) with central composite design (CCD). Four significant variables were found to influence bioethanol yield; initial pH of fermentation medium, substrate concentration, salt content and organic nitrogen concentration with statistically significant effect (p < 0.05) on bioethanol production. The significant factor was then analyzed using central composite design (CCD). The optimum conditions for bioethanol production were substrate concentration at 34.5 g/L, pH 7.61, and organic nitrogen concentration at 6.42 g/L in which giving ethanol yield approximately 1.00 mol/mol. In addition, batch ethanol fermentation in a 2 L bioreactor was performed at the glycerol concentration of 20 g/L, 35 g/L and 45 g/L, respectively. The ethanol yields obtained from all tested glycerol concentrations were approaching theoretical yield when the batch fermentation was performed at optimized conditions. (C) 2014 Published by Elsevier Ltd.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据