期刊
RENEWABLE ENERGY
卷 62, 期 -, 页码 522-526出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.renene.2013.08.016
关键词
Oleaginous red yeast; Amylases-producing yeast; Lipid; Co-cultures; Cassava starch
资金
- National Natural Science Foundation of China [31070029]
Some strains of Rhodosporidium toruloides can produce high concentrations of single cell oil. However, this oleaginous yeast does not produce amylases. Cells of the amylase-producing yeast Saccharomycopsis fibuligera All-c were immobilized using polyvinyl alcohol. The immobilized yeast could produce 325 U/ml of amylase activity within 72 h of incubation. These amylases hydrolyzed cassava starch and the resulting product was converted into single cell oil by R. toruloides 21167. In a 2-1 co-culture bioreactor, a single cell oil yield (64.9% w/w) from a cell mass of R. toruloides 21167 (20.1 g/1) were produced from cassava starch (6.0% w/v). Over 96% of the fatty acids produced were C-16:0, C-18:0, C-58:1 and C-18:2, useful for conversion into biodiesel. (C) 2013 Elsevier Ltd. All rights reserved.
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