期刊
INTERNATIONAL JOURNAL OF LOWER EXTREMITY WOUNDS
卷 14, 期 2, 页码 136-145出版社
SAGE PUBLICATIONS INC
DOI: 10.1177/1534734615569913
关键词
human umbilical cord-derived mesenchymal stem cells; epidermal-like cells; collagen-chitosan scaffold; air-liquid interface; wound healing
资金
- National Basic Science and Development Program [2012CB518103, 2012CB518105]
- 863 Projects of Ministry of Science and Technology of China [2013AA020105, 2012AA020502]
- Military Medical Foundation [AWS11J008]
- National Natural Science Foundation of China [81121004, 81230041]
Human umbilical cord-derived mesenchymal stem cells (UC-MSCs) are multipotent, primitive, and have been widely used for skin tissue engineering. Their transdifferentiation is determined by the local microenvironment. In this study, we investigated the potential epidermal differentiation of UC-MSCs and the formation of epidermis substitutes in a 3-dimensional (3D) microenvironment, which was fabricated by UC-MSCs embedded into collagen-chitosan scaffolds (CCSs) combined with an air-liquid interface (ALI) culture system. Using fluorescence microscope, we observed that UC-MSCs were spindle-shaped and evenly distributed in the scaffold. Methyl thiazolyl blue tetrazolium bromide assay and Live/Dead assay indicated that the CCSs have good biocompatibility with UC-MSCs. Immunohistochemistry and western blotting assay showed that UC-MSCs on the surface of the CCSs were positive for the epidermal markers cytokeratin 19 and involucrin at 14 days. In addition, hematoxylin-eosin staining indicated that multilayered epidermis substitutes were established. The constructed epidermis substitutes were applied to treat full-thickness wounds in rats and proved to promote wound healing. In conclusion, manipulating the 3D microenvironment is a novel method for inducing the epidermal differentiation of MSCs to engineer epidermal substitutes, which provides an alternative strategy for skin tissue engineering.
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