期刊
REGULATORY PEPTIDES
卷 160, 期 1-3, 页码 42-48出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.regpep.2009.12.013
关键词
Basophil; Secretoneurin; Substance P; Chemotaxis; Neurogenic inflammation
资金
- Verein fur tumorforschung - Pneumologie
The sensory neuropeptides secretoneurin (SN) and substance P (SP) are involved in neurogenic inflammatory processes as they occur in bronchial asthma or allergic rhinitis. A possible interaction with basophils has not been reported to date. Basophils were isolated from healthy donors by magnetic cell sorting technique and migration was explored using Boyden microchemotaxis chambers. SN [10(-8)M] and Sp [10(-6) to 10(-8)M] proved to be chemoattractants equally potent to FMLP [10(-8) M] or LPS [10 pg/ml]. Specific anti-SN antibodies and a trypsinization preparation of SN were used to determine the specificity of the SN effect on basophils. The preincubation of basophils with neurokinin-1 (NK-1) or -2 (NK-2) receptor antagonists revealed the SP effect to act via NK-1 receptors in basophils. In addition, we were able to show phosphodiesterases and phosphoinositide-3 kinases to be engaged in the downstream signalling pathway. Our observations reveal for the first time a link between basophils, which are engaged in allergic processes, and the neuropeptides SN and SP. Furthermore, our data might suggest phosphodiesterases or phosphoinositide-3 kinases to be new therapeutic targets for the treatment of allergic diseases such as asthma or allergic rhinitis. (C) 2009 Elsevier B.V. All rights reserved.
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