期刊
REGENERATIVE MEDICINE
卷 6, 期 5, 页码 569-582出版社
FUTURE MEDICINE LTD
DOI: 10.2217/RME.11.48
关键词
biomaterial; cardiac patch; cardiac regeneration; collagen; mesenchymal stem cells
资金
- NHLBI NIH HHS [R21 HL089679-01A2S1, R21 HL089679-02, R21 HL089679] Funding Source: Medline
Aims: Stem cell transplantation holds promise as a therapeutic approach for the repair of damaged myocardial tissue. One challenge of this approach is efficient delivery and long-term retention of the stem cells. Although several synthetic and natural biomaterials have been developed for this purpose, the ideal formulation has yet to be identified. Materials & methods: Here we investigate the utility of a nondenatured, noncrosslinked, commercially available natural biomaterial (TissueMend (R) [TEI Biosciences, Boston, MA, USA]) for delivery of human mesenchymal stem cells (MSCs) to the murine heart. Results: We found that MSCs attached, proliferated and migrated within and out of the TissueMend matrix in vitro. Human MSCs delivered to damaged murine myocardium via the matrix (2.3 x 10(4) +/- 0.8 x 10(4) CD73(+) cells/matrix) were maintained in vivo for 3 weeks and underwent at least three population doublings during that period (21.9 x 10(4) +/- 14.4 x 10(4) CD73(+) cells/matrix). In addition, collagen within the TissueMend matrix could be remodeled by MSCs in vivo, resulting in a significant decrease in the coefficient of alignment of fibers (0.12 +/- 0.12) compared with the matrix alone (0.28 +/- 0.07), and the MSCs were capable of migrating out of the matrix and into the host tissue. Conclusion: Thus, TissueMend matrix offers a commercially available, biocompatible and malleable vehicle for the delivery and retention of stem cells to the heart.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据