期刊
REGENERATIVE MEDICINE
卷 6, 期 1, 页码 81-93出版社
FUTURE MEDICINE LTD
DOI: 10.2217/RME.10.87
关键词
articular cartilage; collagen; decellularized; extracellular matrix; osteoarthritis; proteoglycan; stem cell; tissue engineering
资金
- Osteotech Inc.
- NIH [AR48852, AR48182, AR055042, AG15768, AR50245]
- National Science Council, Taiwan [NSC 97-2320-B-002-010]
- National Taiwan University Hospital [98-N1198]
- NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES [R41AR055042, R01AR048182, R01AR048852, P01AR050245] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE ON AGING [R01AG015768] Funding Source: NIH RePORTER
Aim: To investigate the cell growth, matrix accumulation and mechanical properties of neocartilage formed by human or porcine articular chondrocytes on a porous, porcine cartilage-derived matrix (CDM) for use in cartilage tissue engineering. Materials & methods: We examined the physical properties, cell infiltration and matrix accumulation in different formulations of CDM and selected a CDM made of homogenized cartilage slurry as an appropriate scaffold for long-term culture of human and porcine articular chondrocytes. Results: The CDM scaffold supported growth and proliferation of both human and porcine chondrocytes. Histology and immunohistochemistry showed abundant cartilage-specific macromolecule deposition at day 28. Human chondrocytes migrated throughout the CDM, showing a relatively homogeneous distribution of new tissue accumulation, whereas porcine chondrocytes tended to form a proteoglycan-rich layer primarily on the surfaces of the scaffold. Human chondrocyte-seeded scaffolds had a significantly lower aggregate modulus and hydraulic permeability at day 28. Conclusions: These data show that a scaffold derived from native porcine articular cartilage can support neocartilage formation in the absence of exogenous growth factors. The overall characteristics and properties of the constructs depend on factors such as the concentration of CDM used, the porosity of the scaffold, and the species of chondrocytes.
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