期刊
REACTIVE & FUNCTIONAL POLYMERS
卷 70, 期 7, 页码 471-476出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.reactfunctpolym.2010.03.005
关键词
Carbohydrate; Boronic acid; Biotin; Cyanoxyl-mediated free-radical; polymerization; Glyco-affinity; MALDI-mass spectrometry
资金
- American Health Assistance Foundation [AHAF-H2007027]
- Cleveland State University
A biotin chain-end functionalized boronic acid-containing polymer (biotin boropolymer) as an oriented glyco-affinity macroligand for efficient carbohydrate and glycoconjugate purification and identification application was described. Briefly, the biotin boropolymer was synthesized via a biotin derivated arylamine initiated cyanoxyl-mediated free-radical polymerization in one-pot fashion. The specific streptavidin binding capacity of biotin boropolymer was confirmed by streptavidin-HABA assay, while the specific carbohydrate binding capacity of biotin boropolymer was evaluated by Alizarin Red S binding assay. Efficient glyco-capturing followed by direct MALDI mass spectrometry identification of the captured carbohydrate was demonstrated by using magnetic bead functionalized with the biotin boropolymer via streptavidin/biotin interaction. (C) 2010 Elsevier Ltd. All rights reserved.
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