期刊
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
卷 33, 期 -, 页码 75-85出版社
WILEY
DOI: 10.1002/rcm.8258
关键词
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资金
- CRC 1052
- German Research Council CRC TRR67
- NIH [R01 GM080403, R01 GM073151]
- NSF [CHE 1305874]
Rationale The most frequently occurring phthalate, di(2-ethylhexyl) phthalate (DEHP), causes adverse effects on glucose homeostasis and insulin sensitivity in several cell models and epidemiological studies. However, thus far, there is no information available on the molecular interaction of phthalates and one of the key regulators of the metabolism, the peroxisome proliferator-activated receptor gamma (PPAR gamma). Since the endogenous ligand of PPAR gamma, 15-deoxy-delta-12,14-prostaglandin J(2) (15 Delta-PGJ(2)), features structural similarity to DEHP and its main metabolites produced in human hepatic metabolism, mono(2-ethylhexyl) phthalate (MEHP) and mono(2-ethyl-5-oxohexyl) phthalate (MEOHP), we tested the hypothesis of direct interactions between PPAR gamma and DEHP or its transformation products. Methods Hydrogen/deuterium exchange mass spectrometry (HDX-MS) and docking were conducted to obtain structural insights into the interactions and surface plasmon resonance (SPR) analysis to reveal information about binding levels. To confirm the activation of PPAR gamma upon ligand binding on the cellular level, the GeneBLAzer (R) bioassay was performed. Results HDX-MS and SPR analyses demonstrated that the metabolites MEHP and MEOHP, but not DEHP itself, bind to the ligand binding pocket of PPAR gamma. This binding leads to typical activation-associated conformational changes, as observed with its endogenous ligand 15 Delta-PGJ(2). Furthermore, the reporter gene assay confirmed productive interaction. DEHP was inactive up to a concentration of 14 mu M, while the metabolites MEHP and MEOHP were active at low micromolar concentrations. Conclusions In summary, this study gives structural insights into the direct interaction of PPAR gamma with MEHP and MEOHP and shows that the DEHP transformation products may modulate the lipid metabolism through PPAR gamma pathways.
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