期刊
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
卷 28, 期 22, 页码 2427-2436出版社
WILEY-BLACKWELL
DOI: 10.1002/rcm.7036
关键词
-
资金
- DAPA
- ADD
- National Research Foundation of Korea [2013-056331, 2013-056267]
- Creative Fusion Research Program through the Creative Allied Project - Korea Research Council of Fundamental Science and Technology [CAP-12-1]
RATIONALEThe solid matrices used for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) enable the analysis of small molecules by preventing fragmentations of organic matrix molecules in the low mass-to-charge ratio (m/z) range. In this work, a nylon nanoweb with TiO2 particles was developed as a solid matrix for MALDI-TOFMS to improve the low intensities of mass peaks, narrow detection ranges and low signal-to-noise levels. METHODSThe nylon nanoweb with TiO2 particles was prepared by simultaneously electrospinning a nylon nanoweb and electrospraying TiO2 nanoparticles measuring 25nm in diameter to form TiO2 spheres 300nm in diameter. RESULTSMS of multiple analytes was demonstrated in the low molecular weight range using eight amino acids. Additionally, leucine-enkephalin (555.6g/mol) and cyclic citrullinated peptide (1668g/mol) were used as model analytes to test the feasibility of a nylon nanoweb containing TiO2 particles as a solid matrix for MALDI-TOFMS. CONCLUSIONSThe nylon nanoweb with TiO2 particles can be applied for the detection of volatile small molecule analytes in the m/z ratio range of small molecules. Copyright (c) 2014 John Wiley & Sons, Ltd.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据