期刊
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
卷 23, 期 15, 页码 2316-2320出版社
JOHN WILEY & SONS LTD
DOI: 10.1002/rcm.4150
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资金
- US National Science Foundation [0541740]
- US Environmental Protection Agency [R 833778]
- Direct For Biological Sciences
- Division Of Environmental Biology [0946685] Funding Source: National Science Foundation
- Direct For Biological Sciences
- Division Of Integrative Organismal Systems [0541740] Funding Source: National Science Foundation
Comparative equilibration has been proposed as a methodological approach for determining the hydrogen isotopic composition (delta D) of non-exchangeable hydrogen in complex organic materials, from feathers to blood and soils. This method depends on using homogenized standards that have been previously calibrated for their delta D values of non-exchangeable H, that are compositionally similar to unknown samples, and that span an appropriate isotopic range. Currently no certified organic reference materials with exchangeable H exist, and so isotope laboratories have been required to develop provisional internal calibration standards, such as the keratin standards currently used in animal migration studies. Unfortunately, the isotope ratios of some samples fall outside the range of keratin standards currently used for comparative equilibration. Here we tested a set of five homogenized keratin powders as well as feathers from Painted Buntings and Dark-eyed Juncos to determine the effects of extrapolating comparative equilibration normalization equations outside the isotopic range of keratin standards. We found that (1) comparative equilibration gave precise results within the range of the calibration standards; (2) linear extrapolation of normalization equations produced accurate delta D results to similar to 40 parts per thousand outside the range of the keratins standards used (-187 to -108); and (3) for both homogenized keratin powders and heterogeneous unknown samples there was no difference in variance between samples within and outside the range of keratin standards. This suggested that comparative equilibration is a robust and practical method for determining the delta D of complex organic matrices, although caution is required for samples that fall far outside the calibration range. Copyright (C) 2009 John Wiley & Sons, Ltd.
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