期刊
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
卷 22, 期 8, 页码 1087-1093出版社
WILEY
DOI: 10.1002/rcm.3450
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A rapid, sensitive and selective method has been developed for the analysis of alpha-dicarbonyls using a readily ionizable compound, 9-(3,4-diaminophenyl)acridine (DAA), as a reactive matrix (derivatizing agent and ionization efficiency enhancer), by reactive matrix laser desorption/ionization time-of-flight mass spectrometry (RM-LDI-TOF MS). The reaction between the DAA and alpha-dicarbonyls resulted exclusively in formation of vacuum-stable dicarbonyl-quinoxaline acridine derivatives that were found to possess excellent ionization efficiency in positive ion mode, without the need to use an additional matrix. The a-dicarbonyls used as test compounds included methylglyoxal, dimethylglyoxal, and diphenylglyoxal. Both one-pot and rapid on-plate chemical modification approaches were employed with no extraction or purification necessary. The approach is particularly suitable for high-throughput analysis. The method was found to be selective and specific, with alpha-dicarbonyls unequivocally identified, even in complex matrices, e.g. beer. The figures of merit: relative standard deviation (RSD) 6.9-17%, (n = 4); limit of detection (LOD): <= 0.3 ng mL(-1) for the three standards tested using the one-pot derivatization method; and a good linear calibration curve using an internal standard derivatized in situ (R-2 >= 0.979), demonstrate the applicability of the technique and its utility in improving the sensitivity and precision of the LDI analysis of small molecules. Copyright (0 2008 John Wiley & Sons, Ltd.
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