The effect of fullerenol C60(OH)∼30 on the alcohol dehydrogenase activity irradiated with X-rays

In the present study the effect of X-irradiation on the alcohol dehydrogenase (ADH) activity in the presence of nanoparticles of fullerenol C-60(OH)(similar to 30) under aerobic conditions was investigated in order to assess the potential radioprotective properties of fullerenol. Fullerenol at 75 mg/mL decreased the radiation yield of inactivation of ADH irradiated with fullerenol by 20% comparing to ADH irradiated without fullerenol. Under conditions used during irradiation, 50% of (OH)-O-center dot radicals could react with fullerenol and 50% could react with ADH. Thus, it can be assumed that protective effect of fullerenol on the radiation inactivation of ADH was mostly due to scavenging (OH)-O-center dot radicals by fullerenol. Moreover, fullerenol did not protect against post-irradiation damage as the Ginact for ADH irradiated with fullerenol was still 20% lower than for ADH irradiated without fullerenol after 24 h from irradiation. Additionally, fullerenol at 75 mg/L had no influence on the activity of unirradiated ADH up to 24 h. We concluded that fullerenol C-60(OH)(similar to 30) protected ADH against radiation inactivation due to simple competition for the (OH)-O-center dot radicals and did not modify its activity by association with the protein as it was proved in our previous papers for erythrocyte membrane proteins. (C) 2013 Elsevier Ltd. All rights reserved.