期刊
RADIATION PHYSICS AND CHEMISTRY
卷 79, 期 2, 页码 176-184出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.radphyschem.2009.03.068
关键词
X-ray absorption spectroscopy; X-ray microprobe; FTIR microprobe; Raman microprobes; Cells; Tissues
Protein crystallography and NMR spectroscopy took decades to emerge as routine techniques in structural biology. X-ray absorption spectroscopy now has reached a similar stage of maturity for obtaining complementary local structural information around metals in metalloproteins. However, the relatively recent emergence of X-ray and vibrational spectroscopic microprobes that build on these techniques has enabled the structural information obtained from the mature techniques on isolated biomolecules to be translated into in situ structural information from inhomogeneous complex systems, such as whole cells and tissues. (C) 2009 Elsevier Ltd. All rights reserved.
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