期刊
QUARTERLY REVIEWS OF BIOPHYSICS
卷 46, 期 3, 页码 223-264出版社
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S0033583513000048
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资金
- National Science Foundation [CHE 1012685]
- Optical Science and Engineering Program NSF-IGERT
- National Institutes of Health/University of Colorado Biophysics Training [T32 GM-065103]
- Direct For Mathematical & Physical Scien
- Division Of Chemistry [1012685] Funding Source: National Science Foundation
- Direct For Mathematical & Physical Scien
- Division Of Physics [1125844] Funding Source: National Science Foundation
Nearly two decades after Westhof and Michel first proposed that RNA tetraloops may interact with distal helices, tetraloop-receptor interactions have been recognized as ubiquitous elements of RNA tertiary structure. The unique architecture of GNRA tetraloops (N = any nucleotide, R = purine) enables interaction with a variety of receptors, e. g., helical minor grooves and asymmetric internal loops. The most common example of the latter is the GAAA tetraloop-11 nt tetraloop receptor motif. Biophysical characterization of this motif provided evidence for the modularity of RNA structure, with applications spanning improved crystallization methods to RNA tectonics. In this review, we identify and compare types of GNRA tetraloop-receptor interactions. Then we explore the abundance of structural, kinetic, and thermodynamic information on the frequently occurring and most widely studied GAAA tetraloop-11 nt receptor motif. Studies of this interaction have revealed powerful paradigms for structural assembly of RNA, as well as providing new insights into the roles of cations, transition states and protein chaperones in RNA folding pathways. However, further research will clearly be necessary to characterize other tetraloop-receptor and long-range tertiary binding interactions in detail - an important milestone in the quantitative prediction of free energy landscapes for RNA folding.
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