期刊
CELL ADHESION & MIGRATION
卷 10, 期 3, 页码 259-268出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/19336918.2015.1119362
关键词
BMP2; epicardium; migration; Src; TGFR3
类别
资金
- NIEHS NIH HHS [T32 ES007091, P30 ES006694] Funding Source: Medline
- NIGMS NIH HHS [T34 GM008718] Funding Source: Medline
During embryogenesis, the epicardium undergoes proliferation, migration, and differentiation into several cardiac cell types which contribute to the coronary vessels. The type III transforming growth factor- receptor (TGFR3) is required for epicardial cell invasion and development of coronary vasculature in vivo. Bone Morphogenic Protein-2 (BMP2) is a driver of epicardial cell migration. Utilizing a primary epicardial cell line derived from Tgfbr3(+/+) and Tgfbr3(-/-) mouse embryos, we show that Tgfbr3(-/-) epicardial cells are deficient in BMP2 mRNA expression. Tgfbr3(-/-) epicardial cells are deficient in 2-dimensional migration relative to Tgfbr3(+/+) cells; BMP2 induces cellular migration to Tgfbr3(+/+) levels without affecting proliferation. We further demonstrate that Src kinase activity is required for BMP2 driven Tgfbr3(-/-) migration. BMP2 also requires Src for filamentous actin polymerization in Tgfbr3(-/-) epicardial cells. Taken together, our data identifies a novel pathway in epicardial cell migration required for development of the coronary vessels.
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