期刊
PURINERGIC SIGNALLING
卷 8, 期 2, 页码 295-300出版社
SPRINGER
DOI: 10.1007/s11302-011-9285-0
关键词
ATP; Cytotoxicity; Apoptosis; Signaling
资金
- Telethon - Italy [GGP10082]
- Italian Institute of Technology (IIT)
- Slovenian Research Agency ARRS [J3-2376-1540]
On nociceptive neurons, one important mechanism to generate pain signals is the activation of P2X3 receptors, which are membrane proteins gated by extracellular ATP. In this work, we have studied the recovery of recombinant P2X3 receptor expression in human embryonic kidney (HEK) cells. Our data demonstrated that HEK cells were not permissive for stable P2X3 expression, since the significant time-dependent cell loss. In vivo treatment with P2X3 receptor antagonist limited the effect. The expression of a single P2X3 point mutant Y393A, also largely accelerated cell death. We suggest the requirements of a permissive intracellular molecular machinery for appropriate receptor expression. The present report suggests that despite HEK cells are often used as recombinant expression system for the study a variety of receptors function, they represent a limiting permissive environment for P2X3 receptors.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据