期刊
BIOMEDICAL OPTICS EXPRESS
卷 6, 期 5, 页码 1867-1875出版社
OPTICAL SOC AMER
DOI: 10.1364/BOE.6.001867
关键词
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资金
- National Major Scientific Research Program of China [2011CB910401]
- National Nature Science Foundation of China [81127002, 30925013, 91232306]
- Science Fund for Creative Research Group of China [61121004]
- 985 project
Rapid and high-resolution imaging of large tissues is essential in biological research, like brain neuron connectivity research and cancer margins imaging. Here a novel stage-scanning confocal microscopy was developed for rapid imaging of large tissues. Line scanning methods and strip imaging strategy were used to increase the imaging speed. The scientific CMOS was used as line detector in sub-array mode and the optical sectioning ability can be easily adjusted by changing the number of line detectors according to different samples. Fluorescent beads imaging showed resolutions of 0.47 mu m, 0.56 mu m, and 1.56 mu m in the X, Y, and Z directions, respectively, with a 40 x objective lens. A 10 x 10 mm(2) coronal plane with enough signal intensity could be imaged in about 88 sec at a sampling resolution of 0.16 mu m/pixel. Rapid imaging of mouse brain slices demonstrated the applicability of this system in visualizing neuronal details at high frame rate. (C) 2015 Optical Society of America
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