Studies on green synthesized silver nanoparticles using Abelmoschus esculentus (L.) pulp extract having anticancer (in vitro) and antimicrobial applications

Silver nanoparticles (Ag NPs) were successfully synthesized using AgNO3 via an ecofriendly and simple green route using Abelmoschus esculentus (L.) pulp extract at room temperature. The phytochemicals present in A. esculentus (L.) pulp extract were used both as a reducing and a stabilizing agent for the synthesis of Ag NPs. The stabilization of Ag NPs with phytochemicals was justified using Fourier-transform infrared spectroscopy. The size of the as-synthesized Ag NPs was examined using dynamic light scattering and confirmed by transmission electron microscopy. The crystalline nature of Ag NPs had been identified using X-ray diffraction. The present study demonstrated the efficacy of Ag NPs against Jurkat cells in vitro. Our study also showed that the IC50 dose of Ag NPs leads to the increase in intracellular reactive oxygen species and significantly diminished mitochondrial membrane potential, indicating the effective involvement of apoptosis in cell death. The synthesized Ag NPs also exhibited good antimicrobial activity against different gram class bacteria. (C) 2015 The Authors. Published by Elsevier B.V. on behalf of King Saud University.