期刊
PROTOPLASMA
卷 249, 期 4, 页码 1091-1100出版社
SPRINGER WIEN
DOI: 10.1007/s00709-011-0353-7
关键词
Pb toxicity; H2O2 accumulation; Histochemical detection; Membrane integrity; ROS generation; Antioxidant enzymes
资金
- University Grants Commission, New Delhi, India
We examined the effect of Pb2+ (8 and 40 mg l(-1)) on reactive oxygen species generation and alterations in antioxidant enzymes in hydroponically grown wheat at 24, 72, and 120 h after exposure. Pb2+ toxicity was more pronounced on root growth, and it correlated with the greater Pb accumulation in roots. Pb exposure (40 mg l(-1)) enhanced superoxide anion, H2O2, and MDA content in wheat roots by 1.9- to 2.2-folds, 56-255%, and 41-90%, respectively, over the control. Pb-induced loss of membrane integrity was confirmed by the enhanced electrolyte leakage and in vivo histochemical localization. Activities of scavenging enzymes, superoxide dismutases and catalases, enhanced in Pb-treated wheat roots by 1.4- to 5.7-folds over that in the control. In contrast, the activities of ascorbate and guaiacol peroxidases and glutathione reductases decreased significantly, suggesting their non-involvement in detoxification process. The study concludes that Pb2+-induced oxidative damage in wheat roots involve greater H2O2 accumulation and the deactivation of the related scavenging enzymes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据