期刊
PROTIST
卷 165, 期 5, 页码 569-579出版社
ELSEVIER GMBH
DOI: 10.1016/j.protis.2014.07.003
关键词
Encystation; Amoebozoa; Acanthamoeba keratitis; cellulose synthase; cell wall biosynthesis; Polysphondylium pallidum
类别
资金
- Wellcome Trust [090276, 100293]
- BBSRC grant [BB/K000799]
- Biotechnology and Biological Sciences Research Council [BB/K000799/1] Funding Source: researchfish
- Wellcome Trust [100293/Z/12/Z] Funding Source: researchfish
- BBSRC [BB/K000799/1] Funding Source: UKRI
Amoebas and other freely moving protists differentiate into walled cysts when exposed to stress. As cysts, amoeba pathogens are resistant to biocides, preventing treatment and eradication. Lack of gene modification procedures has left the mechanisms of encystation largely unexplored. Genetically tractable Dictyostelium discoideum amoebas require cellulose synthase for formation of multicellular fructifications with cellulose-rich stalk and spore cells. Amoebas of its distant relative Polysphondylium pallidum (Ppal), can additionally encyst individually in response to stress. Ppal has two cellulose synthase genes, DcsA and DcsB, which we deleted individually and in combination. Dcsa-mutants formed fruiting bodies with normal stalks, but their spore and cyst walls lacked cellulose, which obliterated stress-resistance of spores and rendered cysts entirely non-viable. A dcsa-/dcsb- mutant made no walled spores, stalk cells or cysts, although simple fruiting structures were formed with a droplet of amoeboid cells resting on an sheathed column of decaying cells. DcsB is expressed in prestalk and stalk cells, while DcsA is additionally expressed in spores and cysts. We conclude that cellulose is essential for encystation and that cellulose synthase may be a suitable target for drugs to prevent encystation and render amoeba pathogens susceptible to conventional antibiotics. (C) 2014 The Authors. Published by Elsevier GmbH.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据