期刊
PROTIST
卷 162, 期 2, 页码 288-303出版社
ELSEVIER GMBH, URBAN & FISCHER VERLAG
DOI: 10.1016/j.protis.2010.06.005
关键词
Cycloheximide; perialgal vacuole membrane; secondary endosymbiosis; transmission electron microscopy
类别
资金
- Japan Society for the Promotion of Science (JSPS)
- Narishige Zoological Science Award
- Grants-in-Aid for Scientific Research [22657024, 21247010] Funding Source: KAKEN
Treatment of symbiotic alga-bearing Paramecium bursaria cells with a protein synthesis inhibitor, cycloheximide, induces synchronous swelling of all perialgal vacuoles at about 24 h after treatment under a constant light condition. Subsequently, the vacuoles detach from the host cell cortex. The algae in the vacuoles are digested by the host's lysosomal fusion to the vacuoles. To elucidate the timing of algal degeneration, P. bursaria cells were treated with cycloheximide under a constant light condition. Then the cells were observed using transmission electron microscopy. Results show that algal chloroplasts and nuclei degenerated within 9 h after treatment, but before the synchronous swelling of the perialgal vacuole and appearance of acid phosphatase activity in the perialgal vacuole by lysosomal fusion. Treatment with cycloheximide under a constant dark condition and treatment with chloramphenicol under a constant light condition induced neither synchronous swelling of the vacuoles nor digestion of the algae inside the vacuoles. These results demonstrate that algal proteins synthesized during photosynthesis are necessary to maintain chloroplastic and nuclear structures, and that inhibition of protein synthesis induces rapid lysis of these organelles, after which synchronous swelling of the perialgal vacuole and fusion occur with the host lysosomes. (C) 2010 Elsevier GmbH. All rights reserved.
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