期刊
PROTEOMICS CLINICAL APPLICATIONS
卷 8, 期 3-4, 页码 185-194出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/prca.201300053
关键词
Biomarkers; Isobaric labeling; Multiple sclerosis; Tears
资金
- Pro-Visu Foundation
- Swiss Multiple Sclerosis Society
- Swiss National Science Foundation [310030 132705, 31003A 143987]
- Tissot Economic Foundation
- Swiss National Science Foundation (SNF) [31003A_143987, 310030_132705] Funding Source: Swiss National Science Foundation (SNF)
PurposeMultiple sclerosis is the first cause of progressive neurological disability among young adults living in Western countries. Its diagnosis is mostly based on clinical evaluation, neuroimaging, and in some cases cerebrospinal fluid (CSF) analysis, but no definitive diagnostic test exists. We proposed here that the exploration of tears from multiple sclerosis patients could lead to the discovery of new biomarkers. Experimental designThirty multiple sclerosis patients (20% men) recruited to the Geneva University Hospitals were included in our study (mean age SD [years]: 42.4 15.9). Twenty-five control patients (32% men) were also enrolled (mean age +/- SD [years]: 42.7 +/- 15.1). Tears, CSF or blood was collected for each patient. Three independent quantitative (tandem mass tag) experiments were carried out between tears from multiple sclerosis and control patients. Protein verification was performed by Western blot on tears and CSF and by ELISA on serum samples. ResultsCombined proteomics analyses provided 185 identified tear proteins. Among the differential proteins, alpha-1 antichymotrypsin was the only one to be significantly increased in the three experiments with similar ratios (ratios 1.6 to 2.5, p < 0.05). Its tear, CSF and serum elevation were further confirmed by Western blot and ELISA, respectively. Conclusions and clinical relevanceThis study supports the concept that modifications of the tear proteome can reflect biological abnormalities associated with multiple sclerosis and perhaps other inflammatory conditions affecting the CNS. In addition, alpha-1 antichymotrypsin elevation in tear fluid emerges as a promising biomarker for the diagnosis of multiple sclerosis.
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