期刊
PROTEOMICS CLINICAL APPLICATIONS
卷 4, 期 4, 页码 464-478出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/prca.200900189
关键词
Clinical proteomics; Proteome; Standard; Urine
资金
- European Union [LSHM-C7-2006-037093]
- EuroKUP COST Action [BM0702]
- FP7 DECanBio [201333]
- Agence Nationale pour la Recherche [ANR-07-PHYSIO-004-01]
- NIH [DK075868, DK078244]
Purpose: Urine proteomics is emerging as a powerful tool for biomarker discovery. The purpose of this study is the development of a well-characterized real life sample that can be used as reference standard in urine clinical proteomics studies. Experimental design: We report on the generation of male and female urine samples that are extensively characterized by different platforms and methods (CE-MS, LC-MS, LC-MS/MS, 1-D gel analysis in combination with nano-LC MS/MS (using LTQ-FT ultra), and 2-DE-MS) for their proteome and peptidome. In several cases analysis involved a definition of the actual biochemical entities, i.e. proteins/peptides associated with molecular mass and detected PTMs and the relative abundance of these compounds. Results: The combination of different technologies allowed coverage of a wide mass range revealing the advantages and complementarities of the different technologies. Application of these samples in inter-laboratory and inter-platform data comparison is also demonstrated. Conclusions and clinical relevance: These well-characterized urine samples are freely available upon request to enable data comparison especially in the context of biomarker discovery and validation studies. It is also expected that they will provide the basis for the comprehensive characterization of the urinary proteome.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据