期刊
PROTEOMICS CLINICAL APPLICATIONS
卷 2, 期 12, 页码 1658-1669出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/prca.200780146
关键词
2-D DIGE; Biomarkers; cDNA microarray; Cervical cancer; RNAlater
资金
- Barnes-Jewish Hospital Foundation
- NIH [CA094141, CA95713]
- Alvin J. Siteman Cancer Center at Barnes-Jewish Hospital and Washington University School of Medicine
- NCI Cancer Center [P30 CA91842]
- National Institutes of Health
- National Cancer Institute
- Center for Cancer Research
Cervical cancer screening is ideally suited for the development of biomarkers due to the ease of tissue acquisition and the well-established histological transitions. Furthermore, cell and biologic fluid obtained from cervix samples undergo specific molecular changes that can be profiled. However, the ideal manner and techniques for preparing cervical samples remains to be determined. To address this critical issue a patient screening protein and nucleic acid collection protocol was established. RNAlater was used to collect the samples followed by proteomic methods to identify proteins that were differentially expressed in normal cervical epithelial versus cervical cancer cells. Three hundred ninety spots were identified via 2-D DIGE that were expressed at either higher or lower levels (> three-fold) in cervical cancer samples. These proteomic results were compared to genes in a cDNA microarray analysis of microdissected neoplastic cervical specimens to identify overlapping patterns of expression. The most frequent pathways represented by the combined dataset were: cell cycle: G2/M DNA damage checkpoint regulation; aryl hydrocarbon receptor signaling; p53 signaling; cell cycle: G1/S checkpoint regulation; and the ER stress pathway. HNRPA2B1 was identified as a biomarker candidate with increased expression in cancer compared to normal cervix and validated by Western blot.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据