期刊
PROTEOMICS CLINICAL APPLICATIONS
卷 2, 期 6, 页码 892-903出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/prca.200780122
关键词
cell surface protein marker; stem cells
资金
- Intramural NIH HHS [Z01 AG000847-01] Funding Source: Medline
- NHLBI NIH HHS [R01 HL085434-01A2, P50 HL084946-039003, P50 HL084946-019003, P50 HL084946-029003, N01 HV028180, R01 HL085434, P50 HL084946] Funding Source: Medline
The concept of cell-based therapy has been advocated as a novel approach for treating diseases or conditions where regeneration of cells, tissue and/or potentially organs is required. A promising source for cell-replacement therapies is provided by stem cells, but the success of this approach will ultimately rely on the ability to isolate primary stem or progenitor cells. Cell-surface protein markers will play a critical role in this step. Current methodologies for the identification of cell-surface protein markers rely primarily on antibody availability and flow cytometry, but many cell-surface proteins remain undetectable. Proteomic technologies now offer the possibility to specifically identify and investigate the cell-surface subproteome in a quantitative and discovery-driven manner. Once a cell surface protein marker panel has been identified by MS and the antibodies become available, the panel should permit the identification, tracking, and/or isolation of stem or progenitor cells that may be appropriate for therapeutics. This review provides a context for the use of proteomics in discovering new cell-surface markers for stem cells.
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