期刊
PROTEOMICS
卷 14, 期 7-8, 页码 829-838出版社
WILEY
DOI: 10.1002/pmic.201300047
关键词
Focused microwave irradiation; MALDI-imaging mass spectrometry; Metabolic flux; Stable isotope; Technology
资金
- Japan Science and Technology Agency (JST), Exploratory Research for Advanced Technology (ERATO), Suematsu Gas Biology Project, in Tokyo by Japan Society for the Promotion of Science [24500448]
- JST-Precursory Research for Embryonic Science and Technology (PRESTO) program
- Grants-in-Aid for Scientific Research [24500448] Funding Source: KAKEN
Biotransformation of glucose in organs includes multiple pathways, while quantitative evaluation of percentages of its utilization for individual pathways and their spatial heterogeneity in vivo remain unknown. Imaging MS (IMS) and metabolomics combined with a focused microwave irradiation for rapidly fixing tissue metabolism allowed us to quantify and visualize metabolic fluxes of glucose-derived metabolites in the mouse brain in vivo. At 15 min after the intraperitoneal injection of C-13(6)-labeled glucose, the mouse brain was exposed to focused microwave irradiation, which can stop brain metabolism within 1 s. Quantification of metabolic intermediates containing C-13 atoms revealed that a majority of the C-13(6)-glucose was diverted into syntheses of glutamate, lactate, and uridine diphosphate (UDP)-glucose. IMS showed that regions rich in glutaminergic neurons exhibited a large signal of C-13(2)-labeled glutamate. On the other hand, the midbrain region was enriched with an intensive C-13(6)-labeled UDP-glucose signal, suggesting an active glycogen synthesis. Collectively, application of the current method makes it possible to examine the fluxes of glucose metabolism in a region-specific manner.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据