期刊
PROTEOMICS
卷 13, 期 3-4, 页码 558-567出版社
WILEY
DOI: 10.1002/pmic.201200339
关键词
Cell biology; iTRAQ; miRNA; Multiple reaction monitoring; Noncoding RNA; SILAC
资金
- Council of Scientific and Industrial Research (CSIR), New Delhi, India
- NIH roadmap grant for Technology Centers of Networks and Pathways [U54RR020839]
- National Heart, Lung and Blood Institute [HHSN268201000032C]
MicroRNAs (miRNAs) are small noncoding RNAs that play important roles in posttranscriptional regulation of gene expression. Mature miRNAs associate with the RNA interference silencing complex to repress mRNA translation and/or degrade mRNA transcripts. Mass spectrometry-based proteomics has enabled identification of several core components of the canonical miRNA processing pathway and their posttranslational modifications which are pivotal in miRNA regulatory mechanisms. The use of quantitative proteomic strategies has also emerged as a key technique for experimental identification of miRNA targets by allowing direct determination of proteins whose levels are altered because of translational suppression. This review focuses on the role of proteomics and labeling strategies to understand miRNA biology.
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