期刊
PROTEOMICS
卷 12, 期 2, 页码 313-328出版社
WILEY
DOI: 10.1002/pmic.201100409
关键词
Animal proteomics; Pandinus cavimanus; Scorpion; Transcriptome; Toxin peptide; Venom glands; Venomics
资金
- (FWO)-Flanders [OT/05-064, G.330.06]
- Belgian State - Belgian Science Policy [P6/31]
Venom from the scorpion Pandinus cavimanus was obtained by electrical stimulation of the telson (stinger). Total venom was toxic to crickets at 730 mu g and a paralysis or lethal effect was observed at 30 mu g of venom (death at 1.5 mu g/mg of cricket). Electrophysiological analyses showed cytolytic activity of total venom on oocytes at 7 mu g. HPLC allowed separation of the venom components. A total of 38 fractions from total venom were tested on voltage-gated Na+ and K+ channels. Some fractions block K+ currents in different degrees. By using MS analysis, we obtained more than 700 different molecular masses from telson and venom fractions (by LC-MS/MS and MALDI-TOF MS analyses). The number of disulfide bridges of the telson components was determined. A cDNA library from P. cavimanus scorpion was constructed and a random sequencing screening of transcripts was conducted. Different clones were obtained and were analyzed by bioinformatics tools. Our results reveal information about new genes related to some cellular processes and genes involved in venom gland functions (toxins, phospholipases and antimicrobial peptides). Expressed sequence tags from venom glands provide complementary information to MS and reveal undescribed components related to the biological activity of the venom.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据