期刊
PROTEOMICS
卷 11, 期 6, 页码 1125-1134出版社
WILEY-BLACKWELL
DOI: 10.1002/pmic.201000711
关键词
iTRAQ; Mass spectrometry; Quantification; Technology
资金
- Ministerium fur Innovation, Wissenschaft, Forschung und Technologie des Landes Nordrhein-Westfalen
- Bundesministerium fur Bildung und Forschung [31P5800]
Reporter ion-based methods are among the major techniques to quantify peptides and proteins. Two main labels, tandem mass tag (TMT) and iTRAQ, are widely used by the proteomics community. They are, however, often applied as out-of-the-box methods, without thorough quality control. Thus, due to undiscovered limitations of the technique, irrelevant results might be trusted. To address this issue, we here propose a step-by-step quality control of the iTRAQ workflow. From sample preparation to final ratio calculation we provide metrics and techniques assessing the actual effectiveness of iTRAQ quantification as well as a novel method for more reliable protein ratio estimation.
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