期刊
PROTEOMICS
卷 11, 期 4, 页码 535-553出版社
WILEY
DOI: 10.1002/pmic.201000553
关键词
Label-free quantitation; NSAF; Quantitative proteomics; Shotgun proteomics; Spectral counting; Technology
资金
- Macquarie University
- Australian Research Council
In this review we examine techniques, software, and statistical analyses used in label-free quantitative proteomics studies for area under the curve and spectral counting approaches. Recent advances in the field are discussed in an order that reflects a logical workflow design. Examples of studies that follow this design are presented to highlight the requirement for statistical assessment and further experiments to validate results from label-free quantitation. Limitations of label-free approaches are considered, label-free approaches are compared with labelling techniques, and forward-looking applications for label-free quantitative data are presented. We conclude that label-free quantitative proteomics is a reliable, versatile, and cost-effective alternative to labelled quantitation.
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