期刊
PROTEOMICS
卷 11, 期 21, 页码 4122-4138出版社
WILEY
DOI: 10.1002/pmic.201000485
关键词
cDNA microarray; Drought-induced stress; Metabolites; Oryza sativa L.; Plant proteomics
资金
- National Basic Research Program of China (973 Program) [2010CB125901, 2007CB109201]
- National Special Program on Research and Commercialization of Transgenic Plant [2008ZX08012-002, 2009ZX8009-007B, 2009ZX08001-021B]
- National High Technology Research and Development Program of China (863 Program) [2010AA101806]
- National Natural Science Foundation of China [30830071]
- Shanghai Municipal Science and Technology Commission [2009DJ1400501]
We used proteomic analysis to determine the response of rice plant seedlings to drought-induced stress. The expression of 71 protein spots was significantly altered, and 60 spots were successfully identified. The greatest down-regulated protein functional category was translation. Up-regulated proteins were mainly related to protein folding and assembly. Additionally, many proteins involved in metabolism (e. g. carbohydrate metabolism) also showed differences in expression. cDNA microarray and GC-MS analysis showed 4756 differentially expressed mRNAs and 37 differentially expressed metabolites. Once these data were integrated with the proteomic analysis, we were able to elucidate the metabolic pathways affected by drought-induced stress. These results suggest that increased energy consumption from storage substances occurred during drought. In addition, increased expression of the enzymes involved in anabolic pathways corresponded with an increase in the content of six amino acids. We speculated that energy conversion from carbohydrates and/or fatty acids to amino acids was increased. Analysis of basic metabolism networks allowed us to understand how rice plants adjust to drought conditions.
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