期刊
PROTEOMICS
卷 11, 期 16, 页码 3390-3401出版社
WILEY-BLACKWELL
DOI: 10.1002/pmic.201100036
关键词
Cancer; Cell biology; Mass spectrometry; Phosphorylation; Smad4; Transforming growth factor-beta
资金
- Australian Research Council
- National Health and Medical Research Council (Australia)
- Macquarie University
- Australian Government
The transforming growth factor-beta (TGF-beta) signaling pathway progresses through a series of protein phosphorylation regulated steps. Smad4 is a key mediator of the classical TGF-beta signaling pathway; however, reports suggest that TGF-beta can activate other cellular pathways independent of Smad4. By investigating the TGF-beta-regulated phosphoproteome, we aimed to uncover new functions controlled by TGF-beta. We applied titanium dioxide to enrich phosphopeptides from stable isotope labeling with amino acids in cell culture (SILAC)-labeled SW480 cells stably expressing Smad4 and profiled them by mass spectrometry. TGF-beta stimulation for 30 min resulted in the induction of 17 phosphopeptides and the repression of 8 from a total of 149 unique phosphopeptides. Proteins previously not known to be phosphorylated by TGF-beta including programmed cell death protein 4, nuclear ubiquitous casein and cyclin-dependent kinases substrate, hepatoma-derived growth factor and cell division kinases amongst others were induced following TGF-beta stimulation, while the phosphorylation of TRAF2 and NCK-interacting protein kinase are examples of proteins whose phosphorylation status was repressed. This phosphoproteomic screen has identified new TGF-beta-modulated phosphorylation responses in colon carcinoma cells.
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