期刊
PROTEOMICS
卷 11, 期 12, 页码 2363-2376出版社
WILEY
DOI: 10.1002/pmic.201000186
关键词
Biomarkers; Cell biology; Head and neck cancer; 1-D LC-MS/MS; Secretome; Secretory proteins
资金
- Joseph and Mildred Sonshine Centre for Head and Neck Diseases
- Alex and Simona Shnaider Research Laboratory in Molecular Oncology
- Temmy Latner/Dynacare
- Department of Otolaryngology-Head and Neck Surgery, Mount Sinai Hospital, University of Toronto
- Canadian Institutes of Health Research (CIHR)
- Ontario Research and Development Challenge Fund
In search of blood-based biomarkers that would enhance the ability to diagnose head and neck/oral squamous cell carcinoma (HNOSCC) in early stages or predict its prognosis, we analyzed the HNOSCC secretome (ensemble of proteins secreted and/or shed from the tumor cells) for potential biomarkers using proteomic technologies. LC-MS/MS was used to identify proteins in the conditioned media of four HNOSCC cell lines (SCC4, HSC2, SCC38, and AMOSIII); 140 unique proteins were identified on the basis of 5% global false discovery rate, 122 of which were secretory proteins, with 29 being previously reported to be overexpressed in HNOSCC in comparison to normal head and neck tissues. Of these, five proteins including alpha-enolase, peptidyl prolyl isomerase A/cyclophilin A, 14-3-3 zeta, heterogeneous ribonucleoprotein K, and 14-3-3 sigma were detected in the sera of HNOSCC patients by Western blot analysis. Our study provides the evidence that analysis of head and neck cancer cells' secretome is a viable strategy for identifying candidate serological biomarkers for HNOSCC. In future, these biomarkers may be useful in predicting the likelihood of transformation of oral pre-malignant lesions, prognosis of HNOSCC patients and evaluate response to therapy using minimally invasive tests.
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