期刊
PROTEOMICS
卷 10, 期 6, 页码 1150-1159出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/pmic.200900375
关键词
Bioinformatics; MS/MS; Protein identification
资金
- National Heart, Lung, and Blood Institute
- National Institutes of Health [N01-HV-28179, R44HG004537]
- Center for Systems Biology [PM50 GM076547]
- DIVISION OF HEART AND VASCULAR DISEASES [N01HV028179] Funding Source: NIH RePORTER
- NATIONAL CANCER INSTITUTE [R01CA126239] Funding Source: NIH RePORTER
- NATIONAL HUMAN GENOME RESEARCH INSTITUTE [R44HG004537] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [P50GM076547] Funding Source: NIH RePORTER
The Trans-Proteomic Pipeline (TPP) is a suite of software tools for the analysis of MS/MS data sets. The tools encompass most of the steps in a proteomic data analysis workflow in a single, integrated software system. Specifically, the TPP supports all steps from spectrometer output file conversion to protein-level statistical validation, including quantification by stable isotope ratios. We describe here the full workflow of the TPP and the tools therein, along with an example on a sample data set, demonstrating that the setup and use of the tools are straightforward and well supported and do not require specialized informatic resources or knowledge.
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