期刊
PROTEOMICS
卷 10, 期 7, 页码 1391-1400出版社
WILEY
DOI: 10.1002/pmic.200900710
关键词
Data integration; geLC-MS/MS; Metabolic pathways; Microbiology; Nitrogen fixation; Symbiosis
资金
- ETH Zurich
- Swiss National Foundation for Scientific Research
- University of Zurich Research Priority Program Systems Biology/Functional Genomics
Bradyrhizobium japonicum, a gram-negative soil bacterium that establishes an N-2-fixing symbiosis with its legume host soybean (Glycine max), has been used as a symbiosis model system. Using a sensitive geLC-MS/MS proteomics approach, we report the identification of 2315 B. japonicum strain USDA110 proteins (27.8% of the theoretical proteome) that are expressed 21 days post infection in symbiosis with soybean cultivated in growth chambers, substantially expanding the previously known symbiosis proteome. Integration of transcriptomics data generated under the same conditions (2780 expressed genes) allowed us to compile a comprehensive expression profile of B. japonicum during soybean symbiosis, which comprises 3587 genes/proteins (43% of the predicted B. japonicum genes/proteins). Analysis of this data set revealed both the biases and the complementarity of these global profiling technologies. A functional classification and pathway analysis showed that most of the proteins involved in carbon and nitrogen metabolism are expressed, including a complete set of tricarboxylic acid cycle enzymes, several gluconeogenesis and pentose phosphate pathway enzymes, as well as several proteins that were previously not considered to be present during symbiosis. Congruent results were obtained for B. japonicum bacteroids harvested from soybeans grown under field conditions.
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